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棘头梅童鱼的细胞遗传学特征和X1X1X2

发布时间:2018-6-27 20:52:10   点击数:

CytogeneticcharacterizationanddescriptionofanX1X1X2X2/X1X2YsexchromosomesysteminCollichthyslucidus(Richardson,)

ZHANGShoukang1,2,ZHENGJiao1,2,ZHANGJing1,2,WANGZhiyong1,2,WANGYilei1,2,CAIMingyi1,2*

1TheKeyLaboratoryofHealthyMariculturefortheEastChinaSea,MinistryofAgriculture,Xiamen,China

2FisheriesCollege,JimeiUniversity,Xiamen,China

Abstract:ThechromosomesofspinyheadcroakerCollichthyslucidus(Richardson,)werecharacterizedforthefirsttimebyfluorescencestaining,selfgenomicinsituhybridization(self-GISH),andmulticolorfluorescenceinsituhybridization(FISH)with18SrDNA,5SrDNAandtelomericsequenceprobes.Thefemalekaryotypehasexclusively24pairsofacrocentricchromosomes(2n=48a,NF=48),whilethemaleoneconsistsof22pairsofacrocentricchromosomes,2monosomicacrocentricchromosomesandametacentricchromosome(2n=1m+46a,NF=48).ThedifferencebetweenfemaleandmalekaryotypesindicatesthepresenceofasexchromosomeofX1X1X2X2/X1X2Ytype,whereYistheuniquemetacentricchromosomeinthemalekaryotype.AsrevealedbyFISH,5SrDNAand18SrDNAsitesweremappedatsyntenicpositionofthelargestacrocentricchromosome(X1),andtheshortarmsoftheYchromosomeaswell.AnX1-chromosomespecificinterstitialtelomericsignal(ITS)wasdetectedoverlappingthe5SrDNAsites.Inaddition,self-GISHrevealedthattherepetitiveDNAsaccumulatedonalltheputativesexchromosome.Chromosomefusionac







































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